Measuring surface geometry of adherent cells using oblique transillumination
نویسندگان
چکیده
منابع مشابه
Geometry regulates traction stresses in adherent cells.
Cells generate mechanical stresses via the action of myosin motors on the actin cytoskeleton. Although the molecular origin of force generation is well understood, we currently lack an understanding of the regulation of force transmission at cellular length scales. Here, using 3T3 fibroblasts, we experimentally decouple the effects of substrate stiffness, focal adhesion density, and cell morpho...
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Dynamic visualization of the intravascular events leading to the extravasation of leukocytes into tissues by intravital microscopy has significantly expanded our understanding of the underlying molecular processes. In contrast, the detailed observation of leukocyte transendothelial and interstitial migration in vivo has been hampered by the poor image contrast of cells within turbid media that ...
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Wim Pomp, ∗ Koen Schakenraad, 3, ∗ Hayri E. Balcıoğlu, Hedde van Hoorn, Erik H. J. Danen, Roeland M. H. Merks, 5 Thomas Schmidt, and Luca Giomi † Kamerlingh Onnes-Huygens Laboratory, Leiden University, Niels Bohrweg 2, 2333 CA, Leiden, The Netherlands Instituut-Lorentz, Leiden University, P.O. Box 9506, 2300 RA Leiden, The Netherlands Mathematical Institute, Leiden University, P.O. Box 9512, 23...
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Measuring cell death with colorimetric or fluorimetric dyes such as trypan blue and propidium iodide (PI) can provide an accurate measure of the number of dead cells in a population at a specific time; however, these assays cannot be used to distinguish cells that are dying or marked for future death. In many cases it is essential to measure the proliferative capacity of treated cells to provid...
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ژورنال
عنوان ژورنال: Advances in Bioscience and Biotechnology
سال: 2011
ISSN: 2156-8456,2156-8502
DOI: 10.4236/abb.2011.25053